作者：秦勇 马清涌 党晓燕 沙焕臣
【摘要】 目的 观察白藜芦醇对早期重症急性胰腺炎(SAP)大鼠器官组织SOD、MDA的影响，进一步研究白藜芦醇的作用机制。方法 将54只大鼠随机分为三组(假手术组、急性胰腺炎组、白藜芦醇治疗组各18只)。应用逆行胰胆管穿刺注射牛黄胆酸钠制备SAP大鼠模型，白藜芦醇溶液通过阴茎背静脉注射，术后4h剖杀,观察腹水量及胰、肝、肺、肾和肠壁组织的超氧化物歧化酶(SOD)、丙二醛(MDA)和组织学变化及白藜芦醇对其影响。结果 模型组的胰、肺、肝、肾、小肠组织SOD活性降低、而MDA水平增加；白藜芦醇可明显升高胰、肺、肝、肠、肾组织的SOD活性,降低各组织的MDA水平，同时白藜芦醇可减轻大鼠各器官组织病理损害，以胰腺组织为主。结论 氧自由基参与了SAP的病理生理过程。白藜芦醇可提高SAP大鼠多器官组织SOD活性、降低MDA水平而减轻组织脂质过氧化和组织器官功能损害。
【关键词】 重症急性胰腺炎；白藜芦醇；氧自由基；超氧化物歧化酶；丙二醛
Effect of resveratrol on oxygen free radicals of multiple organs in rats with severe acute pancreatitis of early stage
ABSTRACT: Objective To observe the effect of resveratrol on superoxide dismutase and malondialdehyde of multiple organs in rats with severe acute pancreatitis of early stage. Methods A total of fiftyfour rats were divided into three groups (sham group, SAP model group and resveratroltreated group ). SAP model was made by injecting sodium taurocholate 0.1mL/100g to the pancreatic bile duct, and resvoratrol was given intravenously. The rats were sacrificed at four hours after inducing SAP model. The levels of MDA and SOD and histological changes of pancreas, lungs, liver, kidneys and small intestines were measured. Results The levels of MDA in pancreas, lungs, liver, kidneys and small intestine tissues in model group were significantly higher than those in sham group (P<0.05), while the level of SOD was significantly lower in the tissues involved. The levels of SOD in pancreatic and lung tissues in resveratroltreated group were significantly higher than those in model group, while the levels of MDA in pancreas, lungs, liver, kidneys and small intestines were significantly lower in the tissues concerned. Conclusion Oxygen free radicals participate in the process of pathological changes in SAP. Resveratrol might increase SOD activity and decrease MDA level, thus attenuating lipid peroxidation in multiple organs of SAP, especially in pancreas and lung tissues.
KEY WORDS: severe acute pancreatitis; resveratrol; oxygen free radical; superoxide dismutase; malondialdehyde
重症急性胰腺炎(severe acute pancreatitis, SAP)是胰腺内部及周边突然发生的坏死和炎症的病理过程。急性胰腺炎(acute pancreatitis, AP)尤其是SAP常并发胰外器官损伤，其机制至今不清[13]。近10年来，有研究证实氧自由基(oxygen free radicals, OFR)与AP的发生发展有密切关系。白藜芦醇[45](resveratrol, Res)是一种自由基清除剂，可降低自由基的活性和干扰氧化代谢。本实验旨在研究白藜芦醇对早期SAP大鼠多器官组织氧自由基的影响，探讨其作用机制。
1 材料与方法
1.1 实验动物 健康成年雄性SD大鼠54只，体重250-300g，西安交通大学医学院实验动物中心提供。置于实验环境中1周，室温18-22℃。
1.2 实验仪器与试剂 AH6020应用免疫计数器(国营206厂生产)、低温高速离心机(Heraeus instruments, Germany)、牛黄胆酸钠(美国Sigma公司原装生产)，白藜芦醇(西安奥塞斯公司生物工程有限公司)配成相应浓度的溶液(10g/L白藜芦醇混悬液的配制：用前将10mg白藜芦醇加入无菌注射用水1mL中，用时充分摇匀后缓慢注入血管；40g/L牛黄胆酸钠溶液的配制：称取牛黄胆酸钠1g加入无菌注射用水25mL中，低温保存，用时充分摇匀)；超氧化物歧化酶(SOD)、丙二醛(MDA)测定试剂
您现在的位置：